促肾上腺皮质激素对母猪肝中葡萄糖代谢相关酶转录表达及活性的影响

doi:10.11843/j.issn.0366-6964.2018.11.005

Abstract

Abstract:

This study was designed to investigate the effects of adrenocorticotropic hormone (ACTH) on the gene transcription of glucose metabolism-related enzymes and related enzyme activities in the liver of sows, and to explore the molecular mechanism of blood glucose regulation in the liver under the stress condition. Nine healthy multiparous weaned Suhuai sows were selected and randomly divided into treatment group (N=5) and control group (N=4). Sows in treatment group and control group were injected with ACTH and physiological saline, respectively, three times daily for 7 days. The blood samples were collected daily during the experiment; The liver of sows were collected at the end of experiment. The concentrations of cortisol in blood were detected by radioimmunoassay. The levels of blood glucose were measured by biochemical analyzer. The transcript expression of enzymes related to glucose metabolism and glucocorticoid receptor(GR) were detected by real-time quantitative PCR, the activities of enzymes related to glucose metabolism were detected by spectrophotography. The results showed that the concentrations of cortisol and glucose in blood of sows were significantly raised after ACTH injection (P<0.05). ACTH treatment didn't significantly influence the mRNA expression levels of PC, FBP, PCK2, PEKL, PKLR, HK1, HK3, GYS1, PYGL and G6PD in the liver of sows (P>0.05). ACTH treatment significantly increased the mRNA expression levels of G6PC, PCK1 and HK2 in the liver of sows (P<0.05). Furthermore, ACTH treatment significantly increased the enzyme activities of G6PC (P<0.05), and significantly decreased the enzyme activities of PFKL (P<0.05). Compared with the control group,the mRNA expression levels of GR in the ACTH group showed an up-regulation trend, but there were no significant difference between the two groups (P>0.05). These results suggested that ACTH treatment upregulated blood glucose level through activating transcription of the key gluconeogenesis enzymes PCK1, HK2 and G6PC as well as the enzymatic activities of G6PC to promote gluconeogenesis, meanwhile, reducing the enzyme activities of the key glycolysis enzyme PFKL to repress the glycolysis in the liver. Furthermore, ACTH treatment didn't influence the mRNA expression of GR.

CLC Number:

SUN Ye, ZENG Zi-han, JIA Can-can, NING Cai-bo, WU Wang-jun, ZHAO Fang, LIU Hong-lin. The Effects of Adrenocorticotropic Hormone on the Transcript Expression and Activities of Glucose Metabolism-related Enzymes in the Liver of Sows[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(11): 2340-2348.

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The Effects of Adrenocorticotropic Hormone on the Transcript Expression and Activities of Glucose Metabolism-related Enzymes in the Liver of Sows

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